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KMID : 0603820130190020083
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Journal of Experimental & Biomedical Science
2013 Volume.19 No. 2 p.83 ~ p.89
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Parkin-induced Decrease of β-catenin is Mediated by Protein Kinase C in TNF-α-treated HeLa Cells
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Lee Min-Ho
Jung Byung-Chul
Kim Sung-Hoon
Lee Ju-Yeon
Jung Dong-ju
Cho Jang-Eun
Rhee Ki-Jong
Kim Yoon-Suk
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Abstract
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Parkin is a protein known to have tumor suppressive functions. In a previous study, we determined that Parkin expression restores susceptibility to TNF-α-induced death in HeLa cells. β-catenin is a key protein in the Wnt signaling pathway and excessive activation of the β-catenin pathway can promote cancer development. In this study, we found that β-catenin levels decreased dramatically in Parkin over-expressing HeLa cells treated with TNF-α. We used chemical inhibitors of cell signaling pathways to identify the signaling molecules involved in β-catenin down-regulation. Our results indicate that the PKC inhibitor (RO-31-7549) blocked parkin-induced down-regulation of β-catenin. We also show that Parkin-induced decrease in cell viability in TNF-α-treated HeLa cells is alleviated upon treatment with a PKC inhibitor. Taken together, these results suggest the possibility that β-catenin reduction may be associated with Parkin-induced decrease of cell viability in TNF-α treated HeLa cells.
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KEYWORD
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Parkin, TNF-α, β-catenin, Cervical cancer, Tumor suppressor
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